Application

Anti-5-Hydroxymethylcytosine (5hmC), clone EDL HMC 1A, Cat. No. MABE1093, is a highly specific mouse monoclonal antibody that targets DNA with 5-hydroxymethylcytosine (5hmC) modification and has been tested in Dot Blot and Immunoprecipitation.

Dot Blot Analysis: 0.5 µg/mL from a representative lot detected 0.39-100 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated 5 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

5-Hydroxymethylcytosine (also known as 5-hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). 5hmC is a DNA pyrimidine nitrogen base and is a predominantly stable DNA modification. It is produced by the action of three Ten-eleven translocation 1 (TET1) enzyme on 5mC. Each mammalian cell contains 5hmC, but its levels vary significantly depending on the cell type. Higher levels of 5hmC are found in neuronal cells and its level is shown to increase with age in hippocampus and cerebellum. It is also prevalent in embryonic stems cells and its reduced levels are shown to result in impaired self-renewal of stem cells. In the brain and in embryonic stem cells, 5hmC in enriched in promoters, gene bodies, and intergenic areas near genes and positively correlates with gene expression at these loci. 5hmC appears to play a role to promote gene expression during active demethylation. Significantly lower of 5hmC have been reported in cancer cell lines, melanoma, adenomas, and carcinomas compared to the normal surrounding tissue.

Immunogen

BSA-conjugated 5-hydroxymethylcytidine.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G purified.

Format: Purified

Quality

Evaluated by Dot Blot analysis of DNA cytosine modifications.

Dot Blot Analysis: 0.2 µg/mL of this antibody detected 50 ng of DNA with 5-hydroxymethylcytosine (5hmC), but not unmodified DNA or DNA with only 5-methylcytosine (5mC) modification.

Specificity

Target modification is not species-specific

Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.